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Abstract
The frequency of K-ras point mutation(PM) at codon 12 was studied in 45 patients with
endometrial carcinoma. In vitro amplification of target sequences of DNA extracted from endometrial cancer tissues
by polymerase chain reaction and dot blotting with oligonucleotide hybridization were
performed. Ten of 45 endometrial carcinomas disclosed K-ras PM at codon 12 (22.2%).
Transition from GGT to GAT was most frequent in PM(41.7%). Simultaneously, double
PM (GAT/GCT) were also detected in 2 cases. No relationship appeared to be present
between PM and clinical prognosis such as clinical stage, histological type, histological
grade of differentiation, depth of myometrial invasion, and ascitic cytology. The
positive rates of lymph nude metastasis tended to be higher in the group with positive
PM than in the group without PM. K-ras and C-myc gene amplifications were found in
2 (5.1%) and 3 (7.7%) of 39 cases, respectively. No PM of H-ras at codons 12 and 61
was detected. Our results showed that the PM of K-ras gene at codon 12 was a fairly
common event in genetic abnormality and suggested it would have some role in the progression
of carcinogenesis in endometrial carcinoma.
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Copyright
© 1993 Academic Press. Published by Elsevier Inc. All rights reserved.
